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vectashield mounting medium  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield mounting medium
    Vectashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashield+antifade+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm42898335-91-74-77
    Average 98 stars, based on 22145 article reviews
    vectashield mounting medium - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach.
    Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium “VECTASHIELD® Antifade with DAPI (H-1200) was added, Vector Laboratories” to cover with a slide and seal with commercial nail polish, the images were acquired on a Leica TCS-SP8x confocal microscope, equipped with white laser and high sensitivity detectors (HyD) using a 63X oil immersion objective. ..

    Article Title: Generation of iPSC line NIMHi033-A from an Indian patient with Autism Spectrum Disorder carrying mutation in DYNC1H1 gene
    Article Snippet: .. The following day, cells were washed thrice with 0.1% PBST, incubated with secondary antibodies for one hour, followed by 3 washes with 0.1% PBST, and then mounted with Vectashield Antifade with DAPI (Vector Laboratories, #H-1800-2). .. Images were acquired on a confocal microscope (Molecular Devices).

    Labeling:

    Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach.
    Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium “VECTASHIELD® Antifade with DAPI (H-1200) was added, Vector Laboratories” to cover with a slide and seal with commercial nail polish, the images were acquired on a Leica TCS-SP8x confocal microscope, equipped with white laser and high sensitivity detectors (HyD) using a 63X oil immersion objective. ..

    Microscopy:

    Article Title: Identification of potential new T cell activation molecules: a Bioinformatic Approach.
    Article Snippet: Then the cells were washed 3 times with PBS and incubated for 1 h with primary antibody: α-CD69 (1:20, Abcam, Ab181602), α-CD40 (1:20, Abcam, Ab13545), α-SYT10 (1:20, Novus Biologicals, NBP2-85861), α-RND3 (1:50, Abnova, H00000390-MOI), α-DORA (1:20, EuroScientific, DDX0220P-100), α-GAPDH (1:50, Abcam, Ab233396), αCD53 (1:20, Novus biologicals, NB500393), α-PIN1 (1:20, R&D Systems, MAB2294), Scientific Reports | (2024) 14:22219 9| https://doi.org/10.1038/s41598-024-73003-9 α-MDM4 (1:20, Novus Biologicals, NB100556) dissolved in 1X PBS with 2% FBS. .. After that, 3 washes were performed and then incubated for 1 h with the secondary antibodies α-mouse (1:300, Jackson Immuno Research, 115-454062) and α-rabbit (1:300, Jackson Immuno Research, 111-545-045) labeled with Alexa Fluor® 488, at the end 3 washes with PBS and one more with distilled water, then 15 uL mounting medium “VECTASHIELD® Antifade with DAPI (H-1200) was added, Vector Laboratories” to cover with a slide and seal with commercial nail polish, the images were acquired on a Leica TCS-SP8x confocal microscope, equipped with white laser and high sensitivity detectors (HyD) using a 63X oil immersion objective. ..

    Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues.
    Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goatantirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Vectashield Antifade with DAPI (Vector Laboratories, Newark, CA) and imaged using a Zeiss Observer Z.1 microscope with ZEN 2.6 software (Carl Zeiss Microimaging GmbH, Jena, Germany). ..

    Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues
    Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goat-antirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Vectashield Antifade with DAPI (Vector Laboratories, Newark, CA) and imaged using a Zeiss Observer Z.1 microscope with ZEN 2.6 software (Carl Zeiss Microimaging GmbH, Jena, Germany). ..

    Software:

    Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues.
    Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goatantirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Vectashield Antifade with DAPI (Vector Laboratories, Newark, CA) and imaged using a Zeiss Observer Z.1 microscope with ZEN 2.6 software (Carl Zeiss Microimaging GmbH, Jena, Germany). ..

    Article Title: Comprehensive Approach for Sequential MALDI-MSI Analysis of Lipids, N -Glycans, and Peptides in Fresh-Frozen Rodent Brain Tissues
    Article Snippet: The sections were incubated overnight at +4 °C with the primary antibody anti-Aβ40 (1:500; Invitrogen, Carlsbad, CA) and the next day with Alexa Fluor 488 goat-antirabbit secondary antibody (1:500; A11029, Sigma-Aldrich, Saint Louis, MO) for 1 h at room temperature. .. Slides were mounted with Vectashield Antifade with DAPI (Vector Laboratories, Newark, CA) and imaged using a Zeiss Observer Z.1 microscope with ZEN 2.6 software (Carl Zeiss Microimaging GmbH, Jena, Germany). ..

    other:

    Article Title: Quercetin activates the SIRT6–Nrf2 axis during oxidative stress, modulating DNA repair and ageing-associated markers
    Article Snippet: Slides were blocked with 2.5 % normal horse serum (NHS; Vector Laboratories, USA) for 1 h, then incubated for 1h with either: anti-phospho-histone H2AX (Ser139, mouse mAb, 1:500; Invitrogen, Thermo Fisher Scientific) together with anti-53BP1 (rabbit pAb, 1:500; Invitrogen, Thermo Fisher Scientific) for DSB analysis, or CoraLite 594-conjugated anti-Nrf2 (rabbit pAb, 1:500; Proteintech 16396-1-AP, Manchester, UK) for Nrf2 localisation, following established protocols for oxidative-stress–induced Nrf2 activation [].



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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Image Search Results


    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Morphology, Suspension, Microscopy, Scanning Electron Microscopy, Transmission Assay, Electron Microscopy, Brightfield, Analysis, Confocal, Staining

    Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Suspension, Confocal, Staining, Expressing, Incubation, Widefield, Fluorescence, Microscopy

    BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Expressing, Activity Assay, Suspension, Inhibition, Knock-Out, Confocal, Staining

    CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Activity Assay, Suspension, Confocal, Staining, Expressing, Concentration Assay, MANN-WHITNEY, Drug discovery

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Tissue, Recombinant, Membrane, Gentle, Cell Recovery, Staining, Plasmid Preparation, Saline, Viability Assay, Lysis, Mass Spectrometry, Software